Acute effects of inter-set static stretching on hamstring strength and range of motion (ROM)
Main Article Content
Abstract
Purpose: Inter-set stretching has been proposed as a strategy to influence subsequent performance during resistance exercise, yet its acute effects remain controversial. While some studies suggest potential benefits for flexibility and range of motion (ROM), concerns persist regarding possible impairments in strength output and total training volume (TTV). Methods: Seventeen trained males were randomly assigned to one of two protocols: (a) inter-set static stretching (ISS); and (b) no stretching between sets (WSS). The ISS protocol involved passive hip flexion with the knee extended on the dominant lower limb, consisting of four 45-second sets at maximum perceived discomfort level, performed between exercise sets. In the WSS protocol, participants rested passively between sets without stretching. Results: A main effect for condition was found in the number of repetitions (p = .006), indicating that the ISS protocol led to fewer repetitions compared to the WSS protocol. A main effect for sets was also observed (p ≤ .0001), showing a progressive decrease in repetitions across sets in both protocols. Regarding TTV, the WSS protocol resulted in higher values than the ISS protocol (p = .01), although the effect size was moderate and non-statistically significant (d = .55; CI95% = -0.15 to 1.22). Both protocols promoted a significant acute increase in ROM, with a greater improvement observed in the ISS protocol (p < .05). Conclusion: These results suggest that while ISS may slightly reduce performance measures such as volume and repetition count, it can be effective strategy for enhance range of motion in trained individuals.
Downloads
Article Details
Section

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Each author warrants that his or her submission to the Work is original and that he or she has full power to enter into this agreement. Neither this Work nor a similar work has been published elsewhere in any language nor shall be submitted for publication elsewhere while under consideration by Journal of Human Sport and Exercise (JHSE). Each author also accepts that the JHSE will not be held legally responsible for any claims of compensation.
Authors wishing to include figures or text passages that have already been published elsewhere are required to obtain permission from the copyright holder(s) and to include evidence that such permission has been granted when submitting their papers. Any material received without such evidence will be assumed to originate from the authors.
Please include at the end of the acknowledgements a declaration that the experiments comply with the current laws of the country in which they were performed. The editors reserve the right to reject manuscripts that do not comply with the abovementioned requirements. The author(s) will be held responsible for false statements or failure to fulfill the above-mentioned requirements.
This title is licensed under a Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).
You are free to:
Share — copy and redistribute the material in any medium or format.
Adapt — remix, transform, and build upon the material.
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
-
Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
-
NonCommercial — You may not use the material for commercial purposes.
-
ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
Notices:
- You do not have to comply with the license for elements of the material in the public domain or where your use is permitted by an applicable exception or limitation.
- No warranties are given. The license may not give you all of the permissions necessary for your intended use. For example, other rights such as publicity, privacy, or moral rights may limit how you use the material.
How to Cite
References
Adams, R. (1999). Revised Physical Activity Readiness Questionnaire. Can Fam Physician, 45, 992, 995, 1004-1005.
Alizadeh, S., Daneshjoo, A., Zahiri, A., Anvar, S. H., Goudini, R., Hicks, J. P., Konrad, A., & Behm, D. G. (2023). Resistance Training Induces Improvements in Range of Motion: A Systematic Review and Meta-Analysis. Sports Med, 53(3), 707-722. https://doi.org/10.1007/s40279-022-01804-x
Austin, R. M. (1999). Who should decide how effective cervical cancer screening will be? Acta Cytol, 43(1), 4-6. https://doi.org/10.1159/000330535
Bandy, W. D., Irion, J. M., & Briggler, M. (1997). The effect of time and frequency of static stretching on flexibility of the hamstring muscles. Phys Ther, 77(10), 1090-1096. https://doi.org/10.1093/ptj/77.10.1090
Behm, D. G., Blazevich, A. J., Kay, A. D., & McHugh, M. (2016). Acute effects of muscle stretching on physical performance, range of motion, and injury incidence in healthy active individuals: a systematic review. Appl Physiol Nutr Metab, 41(1), 1-11. https://doi.org/10.1139/apnm-2015-0235
Behm, D. G., & Chaouachi, A. (2011). A review of the acute effects of static and dynamic stretching on performance. Eur J Appl Physiol, 111(11), 2633-2651. https://doi.org/10.1007/s00421-011-1879-2
Bryant, J., Cooper, D. J., Peters, D. M., & Cook, M. D. (2023). The Effects of Static Stretching Intensity on Range of Motion and Strength: A Systematic Review. J Funct Morphol Kinesiol, 8(2). https://doi.org/10.3390/jfmk8020037
Cai, P., Liu, L., & Li, H. (2023). Dynamic and static stretching on hamstring flexibility and stiffness: A systematic review and meta-analysis. Heliyon, 9(8), e18795. https://doi.org/10.1016/j.heliyon.2023.e18795
Chaabene, H., Behm, D. G., Negra, Y., & Granacher, U. (2019). Acute Effects of Static Stretching on Muscle Strength and Power: An Attempt to Clarify Previous Caveats. Front Physiol, 10, 1468. https://doi.org/10.3389/fphys.2019.01468
Chagas, M. H., Bhering, E. L., Bergamini, J. C., & Menzel, H.-J. J. R. B. d. M. d. E. (2008). Comparação de duas diferentes intensidades de alongamento na amplitude de movimento. 14, 99-103. https://doi.org/10.1590/S1517-86922008000200003
Cohen, J. (2013). Statistical power analysis for the behavioral sciences. routledge. https://doi.org/10.4324/9780203771587
Covert, C. A., Alexander, M. P., Petronis, J. J., & Davis, D. S. (2010). Comparison of ballistic and static stretching on hamstring muscle length using an equal stretching dose. J Strength Cond Res, 24(11), 3008-3014. https://doi.org/10.1519/JSC.0b013e3181bf3bb0
Danielsson, A., Horvath, A., Senorski, C., Alentorn-Geli, E., Garrett, W. E., Cugat, R., Samuelsson, K., & Hamrin Senorski, E. (2020). The mechanism of hamstring injuries - a systematic review. BMC Musculoskelet Disord, 21(1), 641. https://doi.org/10.1186/s12891-020-03658-8
Fakhro, M. A., Chahine, H., Srour, H., & Hijazi, K. (2020). Effect of deep transverse friction massage vs stretching on football players' performance. World J Orthop, 11(1), 47-56. https://doi.org/10.5312/wjo.v11.i1.47
Favro, F., Roma, E., Gobbo, S., Bullo, V., Di Blasio, A., Cugusi, L., & Bergamin, M. (2025). The Influence of Resistance Training on Joint Flexibility in Healthy Adults: A Systematic Review, Meta-analysis, and Meta-regression. J Strength Cond Res, 39(3), 386-397. https://doi.org/10.1519/JSC.0000000000005000
Gomes, T. M., Simao, R., Marques, M. C., Costa, P. B., & da Silva Novaes, J. (2011). Acute effects of two different stretching methods on local muscular endurance performance. J Strength Cond Res, 25(3), 745-752. https://doi.org/10.1519/JSC.0b013e3181cc236a
Jackson, A. S., & Pollock, M. L. (1978). Generalized equations for predicting body density of men. Br J Nutr, 40(3), 497-504. https://doi.org/10.1079/BJN19780152
JBB, D. E. C., Brigatto, F. A., Germano, M. D., RM, D. A. C., Teixeira, I., Duarte, R. G., Fellet, L., Braz, T. V., Prestes, J., Marchetti, P. H., Willardson, J. M., & Lopes, C. R. (2022). Acute Effects of Inter-set Stretching on Performance and Metabolic Parameters of Resistance-trained Men. Int J Exerc Sci, 15(4), 231-244. https://doi.org/10.70252/VHXB7598
Junior, R. M., Berton, R., de Souza, T. M., Chacon-Mikahil, M. P., & Cavaglieri, C. R. (2017). Effect of the flexibility training performed immediately before resistance training on muscle hypertrophy, maximum strength and flexibility. Eur J Appl Physiol, 117(4), 767-774. https://doi.org/10.1007/s00421-016-3527-3
Kataura, S., Suzuki, S., Matsuo, S., Hatano, G., Iwata, M., Yokoi, K., Tsuchida, W., Banno, Y., & Asai, Y. (2017). Acute Effects of the Different Intensity of Static Stretching on Flexibility and Isometric Muscle Force. J Strength Cond Res, 31(12), 3403-3410. https://doi.org/10.1519/JSC.0000000000001752
Kato, K., Otoshi, K. I., Tominaga, R., Kaga, T., Igari, T., Sato, R., & Konno, S. I. (2022). Influences of limited flexibility of the lower extremities and occurrence of low back pain in adolescent baseball players: A prospective cohort study. J Orthop Sci, 27(2), 355-359. https://doi.org/10.1016/j.jos.2021.01.008
Kay, A. D., & Blazevich, A. J. (2012). Effect of acute static stretch on maximal muscle performance: a systematic review. Med Sci Sports Exerc, 44(1), 154-164. https://doi.org/10.1249/MSS.0b013e318225cb27
Marin, D. P., Urtado, C. B., Marques, C. G., Serafim, A. I. S., Polito, L. F. T., de Almeida, F. N., Prestes, J., & Otton, R. J. H. M. (2019). Effects of inter-set stretching on acute hormonal and metabolic response: a pilot study. 20(1), 55-61. https://doi.org/10.5114/hm.2019.79218
Medeiros, D. M., Cini, A., Sbruzzi, G., & Lima, C. S. (2016). Influence of static stretching on hamstring flexibility in healthy young adults: Systematic review and meta-analysis. Physiother Theory Pract, 32(6), 438-445. https://doi.org/10.1080/09593985.2016.1204401
Muanjai, P., Jones, D. A., Mickevicius, M., Satkunskiene, D., Snieckus, A., Rutkauskaite, R., Mickeviciene, D., & Kamandulis, S. (2017). The effects of 4 weeks stretching training to the point of pain on flexibility and muscle tendon unit properties. Eur J Appl Physiol, 117(8), 1713-1725. https://doi.org/10.1007/s00421-017-3666-1
Muanjai, P., Jones, D. A., Mickevicius, M., Satkunskiene, D., Snieckus, A., Skurvydas, A., & Kamandulis, S. (2017). The acute benefits and risks of passive stretching to the point of pain. Eur J Appl Physiol, 117(6), 1217-1226. https://doi.org/10.1007/s00421-017-3608-y
Nakamura, M., Ikezu, H., Sato, S., Yahata, K., Kiyono, R., Yoshida, R., Takeuchi, K., & Nunes, J. P. (2021). Effects of Adding Inter-Set Static Stretching to Flywheel Resistance Training on Flexibility, Muscular Strength, and Regional Hypertrophy in Young Men. Int J Environ Res Public Health, 18(7). https://doi.org/10.3390/ijerph18073770
Otsuki, A., Fujita, E., Ikegawa, S., & Kuno-Mizumura, M. (2011). Muscle oxygenation and fascicle length during passive muscle stretching in ballet-trained subjects. Int J Sports Med, 32(7), 496-502. https://doi.org/10.1055/s-0031-1275297
Padilha, U. C., Vieira, A., Vieira, D. C. L., Lima, F. D., Junior, V. A. R., Tufano, J. J., & Bottaro, M. (2019). Could inter-set stretching increase acute neuromuscular and metabolic responses during resistance exercise? Eur J Transl Myol, 29(4), 8579. https://doi.org/10.4081/ejtm.2019.8579
Palmer, T. B., Pineda, J. G., Cruz, M. R., & Agu-Udemba, C. C. (2019). Duration-Dependent Effects of Passive Static Stretching on Musculotendinous Stiffness and Maximal and Rapid Torque and Surface Electromyography Characteristics of the Hamstrings. J Strength Cond Res, 33(3), 717-726. https://doi.org/10.1519/JSC.0000000000003031
Ribeiro, A. S., Romanzini, M., Dias, D. F., Ohara, D., da Silva, D. R., Achour, A., Jr., Avelar, A., & Cyrino, E. S. (2014). Static stretching and performance in multiple sets in the bench press exercise. J Strength Cond Res, 28(4), 1158-1163. https://doi.org/10.1519/JSC.0000000000000257
Rudisill, S. S., Varady, N. H., Kucharik, M. P., Eberlin, C. T., & Martin, S. D. (2023). Evidence-Based Hamstring Injury Prevention and Risk Factor Management: A Systematic Review and Meta-analysis of Randomized Controlled Trials. Am J Sports Med, 51(7), 1927-1942. https://doi.org/10.1177/03635465221083998
Sadler, S. G., Spink, M. J., Ho, A., De Jonge, X. J., & Chuter, V. H. (2017). Restriction in lateral bending range of motion, lumbar lordosis, and hamstring flexibility predicts the development of low back pain: a systematic review of prospective cohort studies. BMC Musculoskelet Disord, 18(1), 179. https://doi.org/10.1186/s12891-017-1534-0
Santos, C. S., Pinto, J. R., Scoz, R. D., Alves, B. M., Oliveira, P. R., Soares, W. J., D. A. Silva Jr RA, J., Vieira, E. R., & Amorim, C. F. (2022). What is the traditional method of resistance training: a systematic review. J Sports Med Phys Fitness, 62(9), 1191-1198. https://doi.org/10.23736/S0022-4707.21.12112-7
Schoenfeld, B. J., Pope, Z. K., Benik, F. M., Hester, G. M., Sellers, J., Nooner, J. L., Schnaiter, J. A., Bond-Williams, K. E., Carter, A. S., Ross, C. L., Just, B. L., Henselmans, M., & Krieger, J. W. (2016). Longer Interset Rest Periods Enhance Muscle Strength and Hypertrophy in Resistance-Trained Men. J Strength Cond Res, 30(7), 1805-1812. https://doi.org/10.1519/JSC.0000000000001272
Schoenfeld, B. J., Wackerhage, H., & De Souza, E. (2022). Inter-set stretch: A potential time-efficient strategy for enhancing skeletal muscle adaptations. Front Sports Act Living, 4, 1035190. https://doi.org/10.3389/fspor.2022.1035190
Schuback, B., Hooper, J., & Salisbury, L. J. P. (2004). A comparison of a self-stretch incorporating proprioceptive neuromuscular facilitation components and a therapist-applied PNF-technique on hamstring flexibility. 90(3), 151-157. https://doi.org/10.1016/j.physio.2004.02.009
Simic, L., Sarabon, N., & Markovic, G. (2013). Does pre-exercise static stretching inhibit maximal muscular performance? A meta-analytical review. Scand J Med Sci Sports, 23(2), 131-148. https://doi.org/10.1111/j.1600-0838.2012.01444.x
Siri, W. E. (1993). Body composition from fluid spaces and density: analysis of methods. 1961. Nutrition, 9(5), 480-491; discussion 480, 492.
Souza, P. A. d., Teixeira, D. R., Corte, J. D., Batista, C. A. d. S., Miranda, H. L., & Paz, G. A. (2020). Acute effect of intra-set static stretching on antagonists versus passive interval on the performance of maximum repetitions of agonists in leg extension machine. Revista Brasileira de Cineantropometria Desempenho Humano, 22, e60225. https://doi.org/10.1590/1980-0037.2020v22e60225
Thomas, E., Ficarra, S., Nunes, J. P., Paoli, A., Bellafiore, M., Palma, A., & Bianco, A. (2023). Does Stretching Training Influence Muscular Strength? A Systematic Review With Meta-Analysis and Meta-Regression. J Strength Cond Res, 37(5), 1145-1156. https://doi.org/10.1519/JSC.0000000000004400
Timmins, R. G., Bourne, M. N., Shield, A. J., Williams, M. D., Lorenzen, C., & Opar, D. A. (2016). Short biceps femoris fascicles and eccentric knee flexor weakness increase the risk of hamstring injury in elite football (soccer): a prospective cohort study. Br J Sports Med, 50(24), 1524-1535. https://doi.org/10.1136/bjsports-2015-095362
Trajano, G. S., Seitz, L., Nosaka, K., & Blazevich, A. J. (2013). Contribution of central vs. peripheral factors to the force loss induced by passive stretch of the human plantar flexors. J Appl Physiol (1985), 115(2), 212-218. https://doi.org/10.1152/japplphysiol.00333.2013
Tran, Q. T., & Docherty, D. (2006). Dynamic training volume: a construct of both time under tension and volume load. J Sports Sci Med, 5(4), 707-713.
Tran, Q. T., Docherty, D., & Behm, D. (2006). The effects of varying time under tension and volume load on acute neuromuscular responses. Eur J Appl Physiol, 98(4), 402-410. https://doi.org/10.1007/s00421-006-0297-3
Van Every, D. W., Coleman, M., Rosa, A., Zambrano, H., Plotkin, D., Torres, X., Mercado, M., De Souza, E. O., Alto, A., Oberlin, D. J., Vigotsky, A. D., & Schoenfeld, B. J. (2022). Loaded inter-set stretch may selectively enhance muscular adaptations of the plantar flexors. PLoS One, 17(9), e0273451. https://doi.org/10.1371/journal.pone.0273451
Wan, X., Li, S., Best, T. M., Liu, H., Li, H., & Yu, B. (2021). Effects of flexibility and strength training on peak hamstring musculotendinous strains during sprinting. J Sport Health Sci, 10(2), 222-229. https://doi.org/10.1016/j.jshs.2020.08.001
Wilson, G. J., Murphy, A. J., & Pryor, J. F. (1994). Musculotendinous stiffness: its relationship to eccentric, isometric, and concentric performance. J Appl Physiol (1985), 76(6), 2714-2719. https://doi.org/10.1152/jappl.1994.76.6.2714
Yagiz, G., Fredianto, M., Ulfa, M., Ariani, I., Agustin, A. D., Shida, N., Moore, E. W. G., & Kubis, H. P. (2024). A retrospective comparison of the biceps femoris long head muscle structure in athletes with and without hamstring strain injury history. PLoS One, 19(2), e0298146. https://doi.org/10.1371/journal.pone.0298146